Math. Model. Nat. Phenom.
Volume 7, Number 5, 2012Immunology
|Page(s)||53 - 64|
|Published online||17 October 2012|
The Use of CFSE-like Dyes for Measuring Lymphocyte Proliferation : Experimental Considerations and Biological Variables
1 Department of Immunology, John Curtin
School of Medical Research, Australian National University,
Canberra, ACT, 2601, Australia
2 School of Medicine, University of Tasmania, Hobart, Tasmania, Australia
⋆ Corresponding author. E-mail:
The measurement of CFSE dilution by flow cytometry is a powerful experimental tool to measure lymphocyte proliferation. CFSE fluorescence precisely halves after each cell division in a highly predictable manner and is thus highly amenable to mathematical modelling. However, there are several biological and experimental conditions that can affect the quality of the proliferation data generated, which may be important to consider when modelling dye dilution data sets. Here we overview several of these variables including the type of fluorescent dye used to monitor cell division, dye labelling methodology, lymphocyte subset differences, in vitro versus in vivo experimental assays, cell autofluorescence, and dye transfer between cells.
Mathematics Subject Classification: 92-02 / 92C37 / 92C99 / 97M60
Key words: Cell proliferation / CFSE / Flow cytometry
© EDP Sciences, 2012
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.